This study aimed to evaluate the antibacterial activity of the hot aqueous extract of Thymus vulgaris on five identified isolates of Staphylococcus aureus, isolated from individuals suffering from chronic tonsillitis, obtained from the Al-Ghari Research Center. Antibiotic susceptibility testing was performed on the five isolates, and the test results showed a significant variation in the strength of the five antibiotics used on the isolates under study. Isolates 1, 2, and 4 showed resistances to the antibiotic doxycycline (CD), while isolates 3 and 5 showed intermediate resistance to the same antibiotic. The results also indicated that isolates 1, 3, 4, and 5 were resistant to the antibiotic OX., The second isolate was intermediately resistant, while the resistance of the isolates under study varied with respect to the other three antibiotics.
Qualitative testing was performed to detect some of the active chemical compounds of this extract. The extract was found to contain phenols, flavonoids, tannins, proteins, and carbohydrates., All isolates showed sensitivity to the hot aqueous extract of thyme at a concentration of 100 mg/ml. All isolates were resistant to the extract at a concentration of 12.5 mg/ml, while the isolates showed varying levels of resistance to the two concentrations of 25 and 50 mg/ml.