Devotion has curdled to the custom of honey owed to its abundant assistances, and with the cumulative necessity to bargain safe, ordinary alternatives to contest certain categories of bacterial corruptions given the high cost of behavior, this study was directed to test honey's facility to kill bacteria in vitro. laboratory. The diffusion method was used to test the effectiveness of Sidr honey and mountain honey at three concentrations each on two forms of bacteria: E. coli and Staphylococcus aureus. The results exhibited that honey was highly effective as an antibacterial agent at all concentrations for mutually types of honey against the bacteria tested. This provides a good suggestion for using honey as an alternative strategy in combating certain modes of bacterial contamination.